Fitc extinction coefficient
WebEXTINCTION COEFFICIENTS AND FLUORESCENCE DATA Calculate extinction coefficient of an oligo by either summing up the extinction coefficients of the … WebFluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original …
Fitc extinction coefficient
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WebCalculate extinction coefficient of an oligo by either summing up the extinction coefficients of the individual bases times their number of ... (6-Fluorescein) 13,700 494 522 Methylene Blue 10,300 81,000 665 Psoralen 16,500 11,000 301 QY 0.07 single-stranded; 0.02 ds, deprotected in ammonia Web• To determine dye-to-protein molar ratio, the extinction molar coefficient (ε) of the unlabeled protein must be known. For more information about protein extinction coefficient (ε), …
WebFITC (Fluorescein-5,6-isothiocyanate) consists of a reactive specie of fluorescein bearing an isothiocyanate reactive group. ... Extinction coefficient (260nm) 13.7 mM-1 cm-1: FITC as an internal modification; MW: 792 g/mol: Excitation maximum: 490 nm: Emission maximum: 525 nm: Extinction coefficient (260nm) WebExtinction coefficient for poly-L-lysine-FITC: E1% = 0.47 (495 nm in 0.02 M Tris buffer, pH 7.4). ... with a molecular weight of 30 to 70 kDa is combined with FITC so that there is an average of one FITC per poly-L-lysine. The fluorescein isothiocyanate is linked to the amino group of the poly-L-lysine by a relatively stable, thiocarbamide ...
Webwhere A max is the absorbance of the FITC-COL I and FITC-COL I-OXI solutions, respectively, measured at 494 nm, the wavelength maximum (λmax) of FITC; D is a dilution factor; ε′ is the molar extinction coefficient of FITC, equal to 70,000 M –1 cm –1; C M is a molar collagen concentration. WebThe extinction coefficient of fluorescein isothiocyanate decreases about 10% on protein conjugation. The fluorescence lifetime (τ) is 3.8 nanoseconds. F36915 consists of a fluorescein solution in 100 mM sodium borate buffer pH 9.5. The concentration of fluorescein is set spectrophotometrically to be equivalent to that of NIST Standard ...
WebThe molar extinction coefficient (ε) for FITC (Fluorescein-5-isothiocyanate) is 73000 cm-1M-1 . Unless otherwise specified, molar extinction coefficient (ε) values are typically …
dallas cowboys rookie linebackerWebAnnexin V-FITC/PI 双染法细胞凋亡检测试剂盒 ， 简单有效 ！. 碘化丙啶 (Propidium Iodide, PI) 是一种核酸染料，它不能透过完整的细胞膜，但 PI 可以透过凋亡中晚期的细胞和死细胞的细胞膜，而使细胞核染上红色染料。 所以， Annexin V 和 PI 匹配使用，可以将处于不同凋亡时期的细胞区分开。 dallas cowboys roster 1982Web53027 Pierce FITC Antibody Labeling Kit, contains sufficient reagents to label and purify 3 × 1mg (2mg/mL) of IgG or similar amounts of other proteins. ... = protein molar extinction coefficient (e.g., the molar extinction coefficient of IgG is ~210,000 M-1: cm-1) • CF = Correction factor: max 280: A A = = 0.3 : dallas cowboys roquan smithWebExtinction Coefficient (ε) 75000 M-1 cm-1: Quantum Yield (φ) 0.92: Closest Laser line: 488 nm: Reactive Group: Isothiocyanate: Reactivity: … birchesgroup.netWebThese measurements were scaled to make the molar extinction coefficient match the value of 92,300cm-1 /M at 500.2nm (Seybold, 1969b). Original Data Extinction Data Fluorescence. The fluorescence emission spectrum of Fluorescein dissolved in basic ethanol. The excitation wavelength was 470nm. The quantum yield of this molecule is … birches group netWebUse the extinction coefficient of FITC and the Beer-Lambert law to calculate the precise concentration of FITC in your sample. Use unreacted FITC as a control (in the same buffer). birches group manilaWebDec 12, 2024 · FITC-labelled protein substrates/peptides, antibodies, peptide hormones are used as specific probes in enzyme kinetics, immunocytochemistry as well as flow cytometry and identification of receptors on target cells, respectively. ... Where ε is molar extinction coefficient, A 280 is absorbance of protein at 280 nm, A 495 is absorbance at 495 nm ... birches green school birmingham